Weinberger Lab Data and Other Resources

Time Lapse Viral Infection

Sixteen Hour Time-Lapse Microscopy Movie of Human Fibroblasts in G0, during Synchronized Infection by Cytomegalovirus (CMV). Originally published in Cell: An Endogenous Accelerator for Viral Gene Expression Confers a Fitness Advantage.

Microfluidics and Acoustic Focusing

A mixture of virus-sized and cell-sized particles (100 nm diameter in red, 10 um diameter in blue) being separated in a microfluidic acoustofluidic chamber (Jung et al. 2015).  In the absence of any acoustic forces (i.e. acoustic field off), red and blue beads overlap.  However, when a transverse acoustic field is applied (indicated in the upper right corner) the large blue particles, which are disproportionally affected by the forces, shift in the flow stream and are separated from the mixture.  In this way viruses and cells can be sorted, enabling purification and/or filtration of virus-infected cultures.

Originally published in Lab on a Chip: Spatial tuning of acoustofluidic pressure nodes by altering net sonic velocity enables high-throughput, efficient cell sorting. (Linked above)

Microwell Time-Lapse Microscopy

Fly-through 3D rendering of microfluidic device designed for long-term imaging of non-adherent cells. An example of the device in action can be see below.

Originally published in Lab on a Chip: Microwell devices with finger-like channels for long-term imaging of HIV-1 expression kinetics in primary human lymphocytes. 

Time-Lapse Microscopy of Lymphocytes

60 hour time-lapse microscopy movie (10 minute intervals) of primary human lymphocytes (activated CD4+ infected with HIV-1 in green, THP1 monocytes in red). Each "finger" is 200 um wide, 100 um long, and 100 um deep and contains about 20 cells on average.

Originally published in Lab on a Chip: Microwell devices with finger-like channels for long-term imaging of HIV-1 expression kinetics in primary human lymphocytes.