Biorepository Core (HIHG)

SAF Order Request Form | Sample Request Form | Sample Submission Form

Extraction of high quality and stable DNA is essential for downstream genomics applications and long term sample storage. The biorepository offers a suite of solutions for extraction from a variety of input types and amounts to assist in any projects specific needs.

• Qiagen Autpure LS
  Automated purification of genomic DNA from large-volume samples
  
  
• Qiagen Symphony
  Automated purification of genomic DNA from small-volume samples and from a wide variety of input sample types.
  
  
• Manual Extraction
  Manual extraction is offered for precious or limited starting material samples, using kits appropriate to the sample type and the volume of input material.

From DNA, RNA, blood, plasma, serum, urine, tissue, cell lines and tissue blocks & slides, the biorepository can meet all your biosample storage needs. Our 4,000 sq. ft. facility has the ability to store at temperatures from ambient to liquid nitrogen.

Pricing is based on sample size and temperature storage needs, starting at $1/sample.

• Brooks Automated SmaRTStore A3+ Sample Archiving System
  Quick access and retrieval of samples at a rate of more than 600 an hour for processing
  
  
• Nautilus LIMS
  Track processing and usage workflows to provide accurate and precise sample data management.

An often overlooked but essential element of sample identification and storage is the design and printing of labels.

Let our experienced scientists help design label solutions for your research without investing in expensive equipment or software training time. We can accommodate label printing on media up to 4 inches wide and 13 inches long for as little as 10 cents per label.

• Brady LabelSoft8 software
  Customize the content and design to fit your unique laboratory needs.

With the ever increasing demand of high-throughput genomics for higher quality DNA in smaller and smaller amounts, the quantitation and qualitation of DNA is paramount to successful research. Using a variety of methods the biorepository can assure that your amount and quality of input is accurate.

• Nanodrop spectrophotometry
  Industry leader in micro-volume UV-Vis quantitation of nucleic acids
  
  
• Qubit fluorometric analysis
  Fluorometer to specifically quantify DNA, RNA, and protein with unprecedented accuracy, sensitivity, and simplicity
  
  
• Agarose gel qualitation
  Every DNA sample is run on an agarose gel to determine the size of the DNA and ensure that it has not been nicked or degraded.

The biorepository tissue culture facility offers a wide range of cell culture services from primary establishment to regrowth from frozen vials.

Specializing in transformation from blood to lymphoblast and primary fibroblast cultures, the facility can manage multiple anchorage and suspension-type cells in most any size or volume to meet the researcher’s needs with pricing starting at $200/sample.

• Lymphoblast
• Fibroblast
• Keritinocyte

As the desire to perform genomic analyses on smaller amounts and on older input material increases, the necessity to accurately and uniformly amplify whole genome DNA grows.

The biorepository uses the Qiagen Repli-G kit with a multiple displacement amplification technique to replicate precious DNA samples with little template bias for an accurate representation of the starting material with pricing starting at $25/sample.

Please do not ship any samples prior to contacting HIHG. All study samples (internal/external) need to be approved for acceptance by Biorepository core prior to shipment. Once samples have been approved for acceptance, please send an email with the Principal Investigator’s name to alert us that samples are on the way to hihgbank@med.miami.edu

Please be sure to follow all applicable International Air Transportation Association (http://www.iata.org/index.htm) regulations for packaging and shipping. We prefer that samples be shipped Monday through Wednesday of the week. Ship via overnight carrier when possible, the time from patient draw and arrival at the bio-repository should be three days or less for UM investigators. If longer times are anticipated to sample arrival due to remote location or field trip scheduling, consideration should be made to use Paxgene® DNA tubes which will stabilize the sample for up to two weeks at room temperature. Please enclose a list with the samples with the Principal Investigator’s name, the sample numbers and amount of sample (concentration, volume and ug) in each tube.

Blood should be shipped at room temperature or on blue ice if we are receiving it within three days of draw, unless it is an ACD tube for transformation then we need to receive it at room temperature. Plasma and serum previously isolated must be shipped on dry ice. DNA should be shipped on dry ice. Saliva in Oragene kits should be shipped at room temperature.

Shipping and Contact Address

HIHG Bio-Repository
1501 NW 10th Ave
BRB Room 448
Miami, FL. 33136
Lab Phone: 305-243-3822
Office Phone: 305-243-3821
Fax: 305-243-2523