Sequencing Core (HIHG)

The Illumina NovaSeq X Plus is the pinnacle of high-throughput sequencing. Surpassing the performance of the NovasSeq 6000, the NovaSeq X offers a 2.5-fold increase in output. Lower cost per gigabase and per read means you can stretch your research budget further.

Highlighted Applications

• Whole Genome Sequencing
• Transcriptome Sequencing
• Targeted gene panels including Human/Mouse Exomes

We also maintain an Illumina MiSeq, generating PE300 reads to facilitate 16S RNA microbiome sequencing and CRISPR screening applications.

The 10x Genomics Chromium X enables single-cell sequencing, unlocking the secrets of individual cells within complex tissues. Profile millions of cells simultaneously, under various conditions, and at genome-scale.

Highlighted Applications

• Single-Cell Gene Expression Profiling
• Chromatin Accessibility Analysis
• Cell Surface Protein Expression

Long-read sequencing (LRS), also known as third-generation sequencing, has rapidly evolved in recent years. The PacBio Revio has a high throughput and ease of use to create exceptionally long reads with very high accuracy with direct methylation detection. This allows for resolution of repetitive regions, structural variation, large-scale genomic rearrangements, inversions, and translocations previously missed by short reads. In addition, RNA based applications allow for isoform detection and quantification in both bulk and single cell applications.

Highlighted Applications:

• Whole Genome Assembly: Complete de novo assembly and genome finishing
• Phased Sequencing: Identify co-inherited alleles and haplotypes
• Epigenetic Modifications: Direct methylation detection
• Isoform sequencing: Full-length isoform sequencing for bulk and single-cell RNA

Long-read sequencing (LRS), also known as third-generation sequencing, has rapidly evolved in recent years. The Oxford Nanopore Technologies PromethION allows for long read sequencing of DNA or RNA with direct base modification detection. This allows for resolution of repetitive regions, structural variation, large-scale genomic rearrangements, inversions, and translocations previously missed by short reads. RNA based applications allow for isoform detection and quantification including chemical modifications of the RNA molecule.

Highlighted Applications

• Whole Genome Sequencing: Complete de novo assembly and genome finishing
• Phased Sequencing: Identify co-inherited alleles and haplotypes
• Epigenetic Modifications: Direct methylation detection
• Direct RNA sequencing: Full-length isoform sequencing with RNA modifications

Researchers can take advantage of the versatility and power of the Illumina NovaSeq X Plus for high throughput and affordable whole genome sequencing. With relatively low input material, highly accurate short read whole genome sequencing is now available across most species.

• Fully automated workflow for reliable and efficient sample processing
• Flexibility in desired sequencing and coverage levels
• Integrated bioinformatics pipeline through variant calling
• Industry standard turnaround times

Solution based targeted capture methods allow researchers to isolate regions of interest from genomic DNA for next-generation sequencing. Off-the-shelf whole exome kits are available, as well as custom designs ranging in size from several to hundreds of genes. We have experience across a variety of the most popular capture reagent suppliers including Agilent, Twist, and Nimblegen.

• Automated sample preparation on liquid handling stations
• Support across a variety of reagent suppliers
• Integrated bioinformatics pipeline through variant calling

The combination of RNA polyA selection or ribo-depletion combined with next generation sequencing has revolutionized gene expression analysis. RNAseq allows for the exploration of functional genomics in human and model organisms and many other types of samples. This includes determination of gene expression patterns, differential expression across conditions, discovery of novel splice variants, and interrogation of long non-coding RNAs and small RNAs.

• Quantity and quality confirmation of total RNA
• Whole transcriptome sample preparations
• Sequencing with the desired number of reads per sample
• Delivery of raw FASTQ files

Quantification of transcripts and chromatin accessibility at single scale resolution is enhancing understanding of a wide variety of conditions. We can utilize the 10x Genomics Chromium X to enable single-cell isolation and library preparation profile millions of cells simultaneously. We can also work with you to utilize other single cell vendors including Parse and others.

• Cell suspension quality control and counting
• Single cell isolation and library prep (RNA, ATAC, or multiome) with Chromium X
• Sequencing in accordance with library preparation to desired number of reads per cell
• Delivery of raw FASTQ files and count matricies

Epigenetic applications available include examination of CpG methylation, histone modifications, chromatin structure and DNA-protein interactions. Methyl-Seq allows researchers to map genome-wide methylation patterns while ChIPseq uses a combination of chromatin immunoprecipitation to quantify in vivo protein-DNA interactions on a genome-wide scale. From your prepared library we can provide high throughput sequencing support for your applications.

• Support for on-boarding of specialized applications
• Sequencing to desired specifications
• Delivery of raw FASTQ files