The iPSC Core is a part of the HIHG Center for Human Molecular Genetics, located on the University of Miami Miller School of Medicine campus. The HIHG iPSC Core provides services for the derivation and characterization of iPSC lines from patient blood and tissue samples. All iPSC lines obtained from the Core are fully validated according to standard operating procedure. Our robust quality control testing protocol assesses sterility, pluripotency, loss of episomal reprogramming vectors, chromosomal abnormalities, and mycoplasma. The HIHG iPSC Core also provides hands-on training and consultation services for the incorporation of iPSC-based approaches in disease modeling, including the development and optimization of protocols and workflows for differentiating a variety of disease-relevant cell types. Additionally, iPSC Core staff have extensive expertise in the genetic manipulation of iPSC lines using CRISPR/Cas9-based gene-editing to create or correct disease mutations.
The iPSC Core facility includes two large, dedicated tissue culture suites, which have multiple full-sized BSL2 hoods and CO2 incubators, a biocontained fluorescent microscope for imaging and sterile manipulation of iPSC cultures, as well as two biocontained dissecting microscopes. We additionally have an Amaxa 4D-Nucleofector, a Miltenyi MACSQuant Analyzer 10 flow cytometer, an Axion Maestro multielectrode array reader, a Tecan Spark 10M multimodal plate reader with Hydroflex unit, Sartorius IncuCyte SX5 and ZOOM live cell imaging systems, and a fully motorized Keyence BZ-X810 all-in-one fluorescent microscope for multicolor optical sectioning microscopy that is additionally equipped with an environmental control chamber.
This facility is located in the same area as the HIHG microscopy core facility which has multiple confocal and fluorescent microscopes, including a Zeiss LSM 780 Laser Scanning Confocal Microscope with Coherent Chameleon Laser, a Zeiss LSM710 Confocal Axio Observer Inverted Automated Microscope, a Zeiss Axio Observer Z.1 Spinning Disk 2 Photon Microscope, a ThermoFisher Arrayscan XTI Infinity High Content Analysis Reader, and a Zeiss Axio Observer Laser Capture Microdissection Microscope.
Services
Take advantage of the HIHG iPSC Core Facilities for high quality services
iPSC line generation
- Generated from peripheral blood mononuclear cells (PBMCs) isolated from whole blood
- Generated from fibroblasts
Pluripotency validation
- Immunocytochemistry
- Alkaline phosphatase staining
- Embroid body formation
- qRT-PCR analysis
- flow cytometry
- hPSC score card
- Sendai virus gDNA PCR
Cell line preservation
- PBMC isolation from blood and cryopreservation
- Mycoplasma testing
- iPSC line cryopreservation
Cell line thawing and expansion
- iPSC lines grown on mouse embryo fibroblasts (MEFs)
- iPSC lines grown on a feeder-free culture, matrigel
Contact Information
For more information or to request a quote, contact us. We welcome all inquiries and look forward to serving your research needs.
Phone: 305-243-8901
Email: bderosa@med.miami.edu