The DRI Flow Cytometry Core Center, directed by Dr. Oliver Umland, provides access to advanced flow cytometry technology to DRI faculty and staff, DRI collaborators, and other investigators within and outside the UM research community. Access may be granted to UM and non-UM researchers whose project align with the DRI mission; to cure diabetes in the fastest and safest way possible.
Staff
Dr. Oliver Umland, PhD, Core Director
Dr. Umland earned this Ph.D. in 2003 from the University of Luebeck, Germany. He has more than 15 years of experience in the field of flow cytometry and immunology. He oversees all core functions, including facility policies, consulting investigators regarding experimental and panel design, data analysis and interpretation as well as instrument QC, maintenance, and operation.
For publications see link below:
https://www.ncbi.nlm.nih.gov/myncbi/1NIGjh-WjmiA7/bibliography/public/
Technology and Services
- Consulting and experimental design
- Instrument and Software training
- Sample analysis on the Cytek Aurora and Beckman Coulter CytoFLEX S analyzers
- High speed cell sorting on the Beckman Coulter Astrios EQ instrument
- Data analysis using SpectroFlo, Kaluza, CytExpert or Summit software
Below is a description of the instruments and analyzers at the DRI Flow Cytometry Center:
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Beckman Coulter MoFlo Astrios EQ cell sorter
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Beckman Coulter CytoFLEX S
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Cytek Aurora
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Beckman Coulter Kaluza analysis software licenses
The 15 parameter, 13 color Beckman Coulter CytoFLEX S benchtop analyzer is equipped with 4 lasers (405nm, 488nm, 561nm, and 640nm) and a 96 well plate reader to facilitate automated high throughput analysis. The system provides high sensitivity and resolution with 7 decades of dynamic range and superior linearity and stability. The instrument is capable of resolving 0.22um particles from noise using SSC off the violet laser.
Services fees
Flow cytometry services are available to investigators on a fee for service basis.