Key Core Functions
- To coordinate proper pre-analytical sample collection and processing procedures with project leaders.
- To perform biochemical assays on samples submitted to the laboratory by investigators in a manner that assures strict quality control both within and between assay runs.
- To report results to project staff in a timely fashion.
- To make available the expertise and technical assistance of the Core for establishing and validating new methodologies to support UM investigators as needs dictate.
Core Description
The Biomarker and Immunoassay Laboratory was established in 1986 by Dr. Ronald B. Goldberg, and is currently co-directed by Drs. Goldberg and Armando J. Mendez.
The laboratory performs assays for the evaluation of metabolic control, hormone levels and disease biomarkers in human blood, urine and saliva samples in support of clinical research studies and in samples from in vitro experimentation (e.g. cell culture medium) in support of pre-clinical studies. The core utilizes validated methods that follow strict quality control procedures to ensure reproducibility of results and common methodology to allow direct comparison between all studies performed by the core. The lab has continuously provided service for the clinical research activities of the DRI and other University of Miami investigators since its inception. The lab is also experienced in the best practices for sample collection and can provide guidance and protocols for standardized sample collection to minimize pre-analytical sample loss and improve stability until sample analysis can be completed.
The laboratory is licensed by the State of Florida and has Clinical Laboratory Improvement Act (CLIA) certification and CLIA guidelines established by for quality control and proficiency testing to ensure reliable, dependable and meaningful results.
Laboratory personnel have extensive experience with chemistry, special chemistry, and immunoassay methods utilizing automated and manual procedures. The laboratory is equipped with a Roche-Cobas 6000 analyzer for automated chemistry and immunoassay testing. In addition, the laboratory is equipped with all instrumentation needed for performing manual immunoassays (ELISA and radioimmunoassay) including microplate spectrophotometers, a microplate fluorimeter, a microplate luminometer, beta and gamma counters for radio-immunoassays and a Luminex 200 analyzer for multiplexed immunoassays.
Laboratory Staff
Rosa Hernandez, MS has over 20 years working in research and clinical chemistry laboratories. Ms Hernandez has been with the Core since 2009 and serves as the laboratory supervisor overseeing the activities of the laboratory staff and daily lab operations. Ms Esperanza Perez has been involved with the laboratory since its inception in 1986 and her current position is senior medical technologist. She has over 35 years of experience in all aspects of clinical chemistry. Ms Blaire Hall has been associated with the laboratory for over 12 years with major responsibility for sample processing and storage and support for the lab staff.
