The Analytical Imaging Core Facility (AICF) supports development and use of novel imaging and analytical approaches to advance scientific research. It provides researchers with access to the state-of-the-art analytical and imaging techniques for cellular and tissue imaging as well as molecular analysis of pathology specimens. Training is provided for investigators, fellows, and technical staff in the proper use of the techniques and instruments.
Technologies
- Leica SP5 confocal microscope Upright and Inverted configurations, 5 lasers each, oil, air and water lenses x63-x5
- High-throughput VS120 Olympus slide scanner, x4-x40 air lenses, 4 channels: DAPI, FITC, TRITC, Cy5
- High-throughput ESSEN IncuCyte X5-x20 lenses, BF, green, red channels
Services
Confocal Imaging
Fixed slides as well as live cells and tissues in a variety of vessels can be imaged. Live cell imaging can be performed using OkoLab chamber with total CO2 and T control.
Animal imaging can be performed using SP5 Upright confocal system. Water-dipping lenses allow for intraocular imaging.
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Confocal Imaging
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Slide Scanning
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TMA Scanning
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Long-Term Imaging (weeks)
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Image Processing
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Laser Microdissection Microscope (LMD)
Contact Information
Marcia Boulina, Ph.D.
Email: mboulina@miami.edu
Phone: 305-243-8436
Location
DRI 6025
1450 N.W. 10th Avenue
Miami, FL 33136